TY - JOUR AU - Döbeli, Heinz PY - 2020/05/27 Y2 - 2024/03/28 TI - Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between JF - CHIMIA JA - Chimia VL - 74 IS - 5 SE - Scientific Articles DO - 10.2533/chimia.2020.363 UR - https://www.chimia.ch/chimia/article/view/2020_363 SP - 363 AB - The present article recapitulates the development of the polyhistidine affinity tag purification principle. Emphasis is laid on events behind the scenes which were never published. The key concept of the method emerged in a team discussion and its further development was driven by the need of Roche in-house projects. ER -