TY - JOUR AU - Cosandey, Vincent AU - Debrot, Fabien AU - Kaeser, Jérémy AU - Marti, Roger AU - Passeraub, Philippe AU - Pétremand, Jannick AU - Prim, Denis AU - Pfeifer, Marc E. PY - 2012/10/31 Y2 - 2024/03/29 TI - Construction of a Peptide Microarray for Auto-anti- body Detection: FH - HES JF - CHIMIA JA - Chimia VL - 66 IS - 10 SE - Columns, Conference Reports DO - 10.2533/chimia.2012.803 UR - https://www.chimia.ch/chimia/article/view/2012_803 SP - 803 AB - Peptide and protein microarrays provide a multiplex approach to identification and quantification of protein–protein interactions (PPI), useful to study for instance antigen–antibody properties. Multivariate serology assays detecting multiple tumor auto-antibodies (TAA) is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA). ER -