High-Resolution and Tandem Mass Spectrometry – the Indispensable Tools of the XXI Century


  • Yury O. Tsybin Biomolecular Mass Spectrometry Laboratory, Ecole Polytechnique Fédérale de Lausanne, EPFL LSMB BCH 4307, CH-1015 Lausanne
  • Luca Fornelli
  • Anton N. Kozhinov
  • Aleksey Vorobyev
  • Sasa M. Miladinovic




Fourier transform ion cyclotron resonance, ft-icr, Liquid chromatography, Mass spectrometry, ms, Peptide, Petroleomics, Protein, Proteomics, Tandem mass spectrometry, ms/ms


Mass spectrometry-based qualitative and quantitative (bio)molecular analysis is a corner stone in the state-of-the-art pipelines in systems biology and environmental sciences. High-resolution and efficient tandem mass spectrometry methods and techniques are the essential analytical capabilities for the in-depth analysis of extremely complex mixtures of (bio)molecules of a very broad dynamic range of concentrations. Here, we briefly review the advantages and limitations of the current mass spectrometry with a focus on resolution, or resolving power, and methods of (bio)molecular fragmentation in the gas phase. We conclude with an outlook that considers possible avenues for further mass spectrometry-based method and technique development, indispensable for advancing the challenging real-life mass spectrometry applications in the XXI century.






Scientific Articles

How to Cite

Y. O. Tsybin, L. Fornelli, A. N. Kozhinov, A. Vorobyev, S. M. Miladinovic, Chimia 2011, 65, 641, DOI: 10.2533/chimia.2011.641.