A Simple Method for High-Throughput Extract Prefractionation for Biological Screening


  • David R. Appleton
  • Antony D. Buss
  • Mark S. Butler MerLion Pharmaceuticals Pte Ltd, 1 Science Park Road, The Capricorn #05-01, Singapore Science Park II, Singapore 117528, Tel: +65 6829 5600, Fax: +65 6829 5601, Email: mark@merlionpharma.com




Automation, High-throughput screening, Natural product, Prefractionation


The screening of crude natural product extracts can be problematic, especially in enzyme/protein-based assays, due to promiscuous inhibitors and interference compounds, while high salt and lipid content can significantly dilute compounds of interest. Described herein is a simple method for high-throughput fractionation of crude extracts that can help address some of these issues. Results obtained indicate a significant improvement in assay hit rates without a reduction of hit quality and show how prefractionation aids the discovery of compounds that would be undetected by screening crude extracts alone.




How to Cite

D. R. Appleton, A. D. Buss, M. S. Butler, Chimia 2007, 61, 327, DOI: 10.2533/chimia.2007.327.