Monitoring Labeling Reactions Using Fluorescence Polarization

FH-HES Universities of Applied Sciences

Authors

  • Yvan Crittin Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland Valais, Route du Rawyl 64, CH-1950 Sion, Switzerland
  • Julien Héritier Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland Valais, Route du Rawyl 64, CH-1950, Switzerland Sion
  • Jean-Manuel Segura Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland Valais, Route du Rawyl 64, CH-1950 Sion, Switzerland. jmanuel.segura@hevs.ch

DOI:

https://doi.org/10.2533/chimia.2013.740

Keywords:

Fluorescence anisotropy, Fluorescence polarization, Fluorescent labeling, Lysozyme

Abstract

Fluorescence techniques are widely applied in protein research owing to their specificity and sensitivity, but require prior fluorescent labeling. Here we show a novel approach to optimize labeling protocols by monitoring labeling reactions using fluorescence polarization: the larger molecular mass of the fluorescent protein conjugate compared to the dye alone results in an increase in fluorescence anisotropy during the reaction. Thereby, labeling of lysozyme with fluorescein isothiocyanate or carboxyfluorescein succinimidyl ester could be monitored and the influence of parameters such as the pH could be quantitatively assessed. Moreover, the impact and kinetics of side reactions such as hydrolysis were determined. This new method is rapid, easy to implement, and generically applicable.

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Published

2013-10-30

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