Artificial Lysosomal Platform to Study Nanoparticle Long-term Stability


  • Ana MilosevicAc
  • Joël Bourquin
  • David Burnand
  • Philipp Lemal
  • Federica Crippa
  • Christophe A. Monnier
  • Laura Rodriguez-Lorenzo
  • Alke Petri-Fink
  • Barbara Rothen-Rutishauser



Artificial lysosomal fluids, Cell uptake, Engineered nanoparticles, Lysosomes, Stability


Abstract: Nanoparticles (NPs) possess unique properties useful for designing specific functionalities for biomedi- cal applications. A prerequisite of a safe-by-design and effective use in any biomedical application is to study NP–cell interactions to gain a better understanding of cellular consequences upon exposure. Cellular uptake of NPs results mainly in the localization of NPs in the complex environment of lysosomes, a compartment which can be mimicked by artificial lysosomal fluid. In this work we showed the applicability of lysosomal fluid as a platform for a fast assessment of gold, iron oxide and silica NP stability over 24 h in a relevant biological fluid, by using multiple analytical methods.




How to Cite

A. MilosevicAc, J. Bourquin, D. Burnand, P. Lemal, F. Crippa, C. A. Monnier, L. Rodriguez-Lorenzo, A. Petri-Fink, B. Rothen-Rutishauser, Chimia 2019, 73, 55, DOI: 10.2533/chimia.2019.55.