Thin-layer Chromatography–Nuclear Magnetic Resonance Spectroscopy – A Versatile Tool for Pharmaceutical and Natural Products Analysis
FH – HES Universities of Applied Sciences
Keywords:Interfaces, Natural products, Nuclear magnetic resonance, Pharmaceutical analysis, Thin layer chromatography, Tlc-ms, Tlc-nmr
AbstractThin-layer chromatography (TLC) is a mature and very established technique, frequently used in many fields of applications ranging from natural product analysis to chemical or pharmaceutical applications. The introduction of a commercially available TLC–MS interface was a major step complementing the ease of use of TLC with structural elucidation power of mass spectrometry (MS). The TLC-MS interface simplifies the workflow dramatically to gain structural information directly from TLC separations. This article describes the potential of TLC-nuclear magnetic resonance spectroscopy (NMR) utilizing the TLC-MS interface to straightforwardly characterize zones of interest by NMR spectroscopy with a focus on quantification of active pharmaceutical ingredients (API) in formulations and identification of active principles in plant extracts.
How to Cite
A. Gössi, U. Scherer, G. Schlotterbeck, Chimia 2012, 66, 347, DOI: 10.2533/chimia.2012.347.
Columns, Conference Reports
Copyright (c) 2012 Swiss Chemical Society
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.