Intracellular Photoactivation and Quantification Using Fluorescence Microscopy: Chemical Tools and Imaging Approaches

Authors

  • Giovanni Bassolino Laboratorium für Organische Chemie, ETH Zürich, HCI G329, Vladimir-Prelog-Weg 3, CH-8093 Zürich, Switzerland
  • Pablo Rivera-Fuentes Laboratorium für Organische Chemie, ETH Zürich, HCI G329, Vladimir-Prelog-Weg 3, CH-8093 Zürich, Switzerland. pablo.rivera-fuentes@org.chem.ethz.ch

DOI:

https://doi.org/10.2533/chimia.2016.796

Keywords:

Fluorescence microscopy, Fluorophores, Imaging, Photoactivation, Uncaging

Abstract

Recent advances in optical microscopy enable the visualization and quantification of biological processes within live cells. To a great extent, these imaging techniques remain limited by the physical properties of the chemical probes that are used as fluorescent tags, detectors and actuators. At the same time, the quantification of concentrations in the intracellular medium is not trivial, but a few approaches that employ optical microscopy have been developed. Herein, we highlight a few examples of how a combination of novel chemical probes and microscopy methods could be used to bring a much-needed quantitative dimension to the field of biological imaging.

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Published

2016-11-30

Issue

Section

Scientific Articles

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