Flow-based Methods in Chemical Peptide and Protein Synthesis
Keywords:Flow chemistry, Ligation, Peptides and proteins, Solid-phase peptide synthesis
AbstractFlow chemistry has emerged as a powerful method for on-demand chemical synthesis and modification of peptides and proteins. Herein, we discuss the characteristics of flow chemistry and how they are applied to various aspects of peptide chemistry. We highlight recent advances in automated flow-based peptide synthesis, which extend the length of peptides routinely accessible to single-domain proteins and allow for the collection of time-resolved synthesis data. Applications of this data for the prediction of synthesis outcome and the potential for the development of more sustainable synthesis methods are also discussed. Finally, we will review solutionphase approaches, including flow-based ligation strategies and peptide cyclization. Throughout this review, the current challenges and potential future developments are highlighted.
How to Cite
K. Schiefelbein, N. Hartrampf, Chimia 2021, 75, 480, DOI: 10.2533/chimia.2021.480.
Copyright (c) 2021 Kevin Schiefelbein, Nina Hartrampf
This work is licensed under a Creative Commons Attribution 4.0 International License.