Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides folium
Keywords:Liquid chromatography, Polyamine alkaloids, Spider venom, Structure elucidation, Tandem mass spectrometry
AbstractThe lyophilized venom of the spider Larinioides folium (Araneidae) was analyzed by high-performance liquid chromatography, coupled on-line to electrospray ionization mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (HPLC-ESI-MS/MS), as well as UV-DAD analysis. In combination with amino acid analysis, high-resolution mass spectrometry and on-column H/D-exchange experiments, the structures of 41 new acyl-polyamines contained in the complex venom of the spider L. folium were elucidated. Data interpretation in detail and the efficiency of this combined set of analytical method is exemplified by the structural elucidation of one of the toxins, namely of LF503a.
How to Cite
S. Eichenberger, L. Bigler, S. Bienz, Chimia 2007, 61, 161, DOI: 10.2533/chimia.2007.161.
Copyright (c) 2007 Swiss Chemical Society
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.