Microchip Isoelectric Focusing Applications

Authors

  • Markéta Vlčková
  • Maria A. Schwarz

DOI:

https://doi.org/10.2533/chimia.2008.244

Keywords:

Electrophoresis, Isoelectric focusing, Microfluidics, Proteomics, Whole-column imaging detection

Abstract

This review summarizes all available publications on isoelectric focusing carried out on planar microfabricated devices. Characteristic features of microchip isoelectric focusing (MC-IEF), such as resolution independence of separation length and compression of pH gradient, are discussed. An overview of materials used for microchip fabrication and developed detection strategies is given. Accomplishments in on-chip coupling of MC-IEF with other electrophoretic separation techniques or with mass spectrometry are briefly described. The review ends with alternative approaches to MC-IEF separation in terms of unusual sample introduction, kinds of support used for IEF separation, and in manner in which the pH gradient is generated.

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Published

2008-04-30