Vitamin E and Vitamin E Acetate Absorption from Self-assembly Systems under Pancreas Insufficiency Conditions

Authors

  • Kornél Nagy Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland
  • Birgit Holst Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland
  • Javier Santos Digestive System Research Unit, Institut de Recerca Vall d'Hebron; Department of Gastroenterology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (Department of Medicine), Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain
  • Martin Kussmann Molecular Biomarkers Core, Nestlé Institute of Health Sciences, Campus EPFL, Quartier de l'innovation, bâtiment H, CH-1015 Lausanne, Switzerland; Ecole Polytechnique Fédérale Lausanne (EPFL), Lausanne, Switzerland; Faculty of Science, Aarhus University, Aarhus, Denmark
  • Laurent Sagalowicz Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland
  • Simone Acquistapace Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland
  • Julie Moulin Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland
  • Maurice Beaumont Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland
  • Juan Ramón Malagelada Digestive System Research Unit, Institut de Recerca Vall d'Hebron; Department of Gastroenterology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (Department of Medicine), Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain
  • Fernando Azpiroz Digestive System Research Unit, Institut de Recerca Vall d'Hebron; Department of Gastroenterology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (Department of Medicine), Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain
  • Valentí Puig-Divi Digestive Diseases Unit, Hospital de Sabadell, Institut Universitari Parc Taulí, Universitat Autònoma de Barcelona, Sabadell, Spain
  • Laura Ramos Digestive System Research Unit, Institut de Recerca Vall d'Hebron; Department of Gastroenterology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (Department of Medicine), Barcelona, Spain
  • Sophie Braga-Lagache Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland
  • Marie-Claude Courtet-Compondu Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland
  • Beatriz Lobo Digestive System Research Unit, Institut de Recerca Vall d'Hebron; Department of Gastroenterology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (Department of Medicine), Barcelona, Spain
  • Gary Williamson School of Food Science and Nutrition, University of Leeds, Leeds, UK

DOI:

https://doi.org/10.2533/chimia.2014.129

Keywords:

Bioavailability, Chronic pancreas insufficiency, Self-assembly systems, Vitamin e

Abstract

We determined the bioavailability of vitamin E from self-assembly structures in patients with diagnosed chronic pancreas insufficiency. Vitamin E solubilized in dispersed inverted bicontinuous cubic phase and in micellar formulation was delivered directly to the small intestine by tube-feeding. A cross-over study with randomization of 6 subjects and 2 treatments including a combined dose of 18 mg (27 IU) of vitamin E (RRR-[5,7-methyl-(2H6)]-?-tocopherol) and 27 mg (27 IU) vitamin E acetate (RRR-[5-methyl-2H3]-?-tocopheryl acetate) was applied over a time period of 1 h. Plasma samples were collected for 56 h and analyzed by liquid chromatography–mass spectrometry. Appearance of labeled tocopherols originating from the treatment started at 25 h and reached Cmax (0.6–4.6 ?M depending on subject) in the 7–9 h window. From the Tmax onwards, both forms of tocopherols diminished slowly to 30–50% of their maxima within 56 h. Strong inter-individual variation was observed in the plasma appearance curves (relative standard deviation varied between 38–45%). No significant discrimination was found between the absorption of free or acetylated forms of deuterated ?-tocopherol confirming that application of acetylated ?-tocopherol provides the same bioavailability as free ?-tocopherol. This observation is valid in both dispersed inverted bicontinuous cubic phase and micellar formulations. Furthermore, since the area-under-the-curve values from cubic phase and from micellar formulations are similar, the cubic phase formulation could represent an alternative delivery system for lipophilic micronutrients in conditions or studies where polysorbate-based micelles cannot be generated.

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Published

2014-03-26

How to Cite

[1]
K. Nagy, B. Holst, J. Santos, M. Kussmann, L. Sagalowicz, S. Acquistapace, J. Moulin, M. Beaumont, J. R. Malagelada, F. Azpiroz, V. Puig-Divi, L. Ramos, S. Braga-Lagache, M.-C. Courtet-Compondu, B. Lobo, G. Williamson, Chimia 2014, 68, 129, DOI: 10.2533/chimia.2014.129.