A Step-by-Step Guide to Study Protein–RNA Interactions
DOI:
https://doi.org/10.2533/chimia.2019.406PMID:
31118123Keywords:
Affinity, Protein–rna complex, Protein–rna interface, Rbd, RbpAbstract
Protein–RNA complex formation is at the center of RNA metabolism and leads to the modulation of protein and RNA functions. We propose here a step-by-step guide to investigate these interactions including the identification of the protein and RNA parts involved in complex formation, the determination of the affinity of the complex and the characterization of the protein–RNA interface at amino acid and nucleotide level. Moreover, we briefly review the methods that are the most often used to obtain this information using primarily examples from our lab and finally mention what we perceive as the next challenges in the field.Downloads
Published
2019-05-29
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Section
Scientific Articles
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Copyright (c) 2019 Swiss Chemical Society
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
[1]
Chimia 2019, 73, 406, DOI: 10.2533/chimia.2019.406.