A Step-by-Step Guide to Study Protein–RNA Interactions

Authors

  • Antoine Cléry
  • Laurent Gillioz
  • Cristina K. X. Nguyen
  • Frédéric H.-T. Allain

DOI:

https://doi.org/10.2533/chimia.2019.406

PMID:

31118123

Keywords:

Affinity, Protein–rna complex, Protein–rna interface, Rbd, Rbp

Abstract

Protein–RNA complex formation is at the center of RNA metabolism and leads to the modulation of protein and RNA functions. We propose here a step-by-step guide to investigate these interactions including the identification of the protein and RNA parts involved in complex formation, the determination of the affinity of the complex and the characterization of the protein–RNA interface at amino acid and nucleotide level. Moreover, we briefly review the methods that are the most often used to obtain this information using primarily examples from our lab and finally mention what we perceive as the next challenges in the field.

Downloads

Published

2019-05-29

Issue

Section

Scientific Articles