Challenges in ESI-MS-based Untargeted Metabolomics
Keywords:Annotation, Capillary electrophoresis, Liquid chromatography, Mass spectrometry, Metabolomics, Supercritical fluid chromatography, Toxicological and doping analysis
Untargeted metabolomics is now widely recognized as a useful tool for exploring metabolic changes taking place in biological systems under different conditions. In this article, we aim to provide a short overview of the liquid-phase separation methods hyphenated to MS to perform untargeted metabolomics of biological samples. Each approach is complemented by up-to-date literature to guide readers, as well as practical information for avoiding or fixing some of the most frequently encountered pitfalls. This article covers mainly data acquisition, but a short discussion is provided regarding signal processing and data treatment, as well as data analysis and its biological interpretation in the context of metabolomic studies.
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Copyright (c) 2022 Serge Rudaz, Elena Tobolkina, Víctor González-Ruiz, Isabel Meister, Miguel De Figueiredo, Davy Guillarme, Julien Boccard
This work is licensed under a Creative Commons Attribution 4.0 International License.