Challenges in ESI-MS-based Untargeted Metabolomics

Authors

  • Serge Rudaz University of Geneva, Swiss Center for Applied Human Toxicology
  • Elena Tobolkina University of Geneva
  • Víctor González-Ruiz University of Geneva, Swiss Center for Applied Human Toxicology
  • Isabel Meister University of Geneva
  • Miguel De Figueiredo University of Geneva
  • Davy Guillarme University of Geneva
  • Julien Boccard University of Geneva, Swiss Center for Applied Human Toxicology

DOI:

https://doi.org/10.2533/chimia.2022.90

Keywords:

Annotation, Capillary electrophoresis, Liquid chromatography, Mass spectrometry, Metabolomics, Supercritical fluid chromatography, Toxicological and doping analysis

Abstract

Untargeted metabolomics is now widely recognized as a useful tool for exploring metabolic changes taking place in biological systems under different conditions. In this article, we aim to provide a short overview of the liquid-phase separation methods hyphenated to MS to perform untargeted metabolomics of biological samples. Each approach is complemented by up-to-date literature to guide readers, as well as practical information for avoiding or fixing some of the most frequently encountered pitfalls. This article covers mainly data acquisition, but a short discussion is provided regarding signal processing and data treatment, as well as data analysis and its biological interpretation in the context of metabolomic studies.

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Published

2022-02-23

How to Cite

[1]
S. Rudaz, E. Tobolkina, González-Ruiz V., I. Meister, M. De Figueiredo, D. Guillarme, J. Boccard, Chimia 2022, 76, 90, DOI: 10.2533/chimia.2022.90.