Covalent Labeling of Fusion Proteins with Chemical Probes in Living Cells
Keywords:O6-alkylguanine-dna alkyltransferase, Fluorescent probes, Protein labeling
AbstractA general method for the specific and covalent labeling of fusion proteins in vivo is described. The approach is based on the unusual mechanism of the human DNA repair protein O6-alkylguanine-DNA alkyl-transferase, which irreversibly transfers an alkyl group from O6-alkylguanine-DNA to one of its cysteine residues. Labeling is possible in bacterial as well as eukaryotic cells and is independent of the nature of the label, thereby opening up new ways to study proteins in vivo.
How to Cite
S. Gendreizig, A. Keppler, A. Juillerat, T. Gronemeyer, H. Pick, H. Vogel, K. Johnsson, Chimia 2003, 57, 181, DOI: 10.2533/000942903777679433.
Copyright (c) 2003 Swiss Chemical Society
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