Pyoverdine Analysis – From High-resolution MS/MS Fragmentation to Ion Mobility Measurements
Keywords:Mass Spectrometry, Pseudomonas, Siderophore, Structural Elucidation
Microorganisms produce iron chelators called siderophores that are a rich source for drug discovery or plant protective agents. Pyoverdines are a class of siderophores from fluorescent Pseudomonas members and consist of different peptide chains specific to each bacterial species. The structural elucidation and characterization of pyoverdines require comprehensive analytical methods as bacterial extracts are complex mixtures. Here, we present a high-throughput UHPLC-MS/MS pipeline and the application of ion mobility spectrometry to facilitate research in the field of medicine and agriculture.
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Copyright (c) 2023 Karoline Rehm, Vera Vollenweider, Rolf Kümmerli, Laurent Bigler
This work is licensed under a Creative Commons Attribution 4.0 International License.